Fig. 5

NS5ATP9 mRNA level in lung tissues of BLM-induced mice at different time points and the optimum concentration of TAF on cells. a NS5ATP9 RNA level in mice sacrificed on day 21, day 28 and day 35. Mice were respectively sacrificed on 21st, 28th, and 35th days, after 3 weeks under different intervention conditions of TAF or PBS separately from day 0, day 7, or day 14; then, the left lung section was excised and lysed, and used for mRNA levels of NS5ATP9. NS5ATP9 was determined by RT-qPCR. b Cells were treated with various concentrations (0.5, 5, 50, 250, 500 μmol/L) of TAF for 24 h or 48 h, and cell viability was measured using the CCK-8 assay. c Cells were serum-starved overnight, before incubation with TGF-β1 (5 ng/ml) or vehicle for 24 h, and then treated with vehicle or TAF with the concentrations of 0.5, 5, 50, 250, and 500 μmol/L. Besides, 24 h after compound dosing, the cells were collected and lysed, and used for detecting mRNA levels of α-SMA and collagen 3α1. Collagen 3α1 and TGF-β1 were determined by RT-qPCR. Statistical significance across intervention groups was determined using one-way ANOVA. #P < 0.05 vs. control group or untreated cells. *P < 0.05 vs. BLM group