Fig. 4

TAF suppressed TGF-β1, collagen 3α1, α-SMA, and fibronectin expression in lung tissues of BLM-treated mice. a Western blot analysis of TGF-β1, collagen 3α1, α-SMA, and fibronectin expression in lung tissues of mice sacrificed on day 21 after administration of saline or BLM; (b) The expression level of the above-mentioned production on day 28; (c) The expression level of the above-mentioned production on day 35. Increased protein levels of the above-mentioned targets were observed in BLM group (BLM + saline) compared with control group (saline + saline) and TAF group (BLM + TAF). All data were analyzed by densitometry and normalized to β-actin. Data were presented as mean ± standard error of the mean (SEM) of at least three separate experiments, and statistical significance across intervention groups was determined using one-way ANOVA. #P < 0.05 vs. control group. *P < 0.05 vs. BLM group