Fig. 1

Expression level of NS5ATP9 in TGF-β1-induced HFL1 cells and its effects on suppressing ECM production. HFL1 cells were transfected transiently with pcDNA3.1/myc-His(−)-NS5ATP9 plasmid or siRNA-NS5ATP9 for 24 h. pcDNA3.1/myc-His(−) and siRNA-NC were used as their negative controls, respectively. Cells without transfection were used as the blank control. The expression level and inhibitory effects on ECM production of NS5ATP9 were analyzed by Western blotting (a-c), while the efficiency of RNA interference was evaluated at mRNA level via RT-qPCR (b). β-actin was used as an internal reference RT-qPCR. The expression level of proteins was evaluated by quantitative ratios shown as the relative optical densities of the bands normalized to β-actin expression. The results were shown as mean ± standard error of mean (SEM), and statistical significance across intervention groups was determined using one-way ANOVA. All assays were performed in triplicates. *P < 0.05 vs. pcNC-NS5ATP9 group, #P < 0.05 vs. siNC-NS5ATP9 group