Fig. 3

IPF derived fibroblasts have high levels of HIF-1α & -2α expression. Representative western blots of HIF1α (a) and HIF2α (d) after 48 h of exposure to hypoxia (1% O2). β-actin was used as a loading control. Each bar represents the mean ± SD of the two fibroblast lines for each group (b, e). Gene expression of HIF1α (c) and HIF2α (f) at basal (0 h) versus 12, 24, 48, 72 and 96 h of exposure to hypoxia (1% O2) of the normal fibroblast and in fibroblast from idiopathic pulmonary fibrosis. Representative immunocytochemistry of HIF2α in IPF fibroblast and in normoxia and after 48 h of exposure to hypoxia (g)