Fig. 2

Basal expression of hypoxia-inducible alpha subunits on IPF derived fibroblasts. Western blots of HIF1α (a), HIF2α (b) & HIF3α (c) in fibroblasts from IPF lungs and controls cultured at 37 °C in an atmosphere of 95% air and 5% CO2, passage 5–7. β-actin was used as a loading control. Densitometric analysis, each bar represents the mean ± SD of 4 different cell lines for each group. *P < 0.05, **P < 0.01 two-tailed Student’s t-test