Fig. 2

Salicylic acid or toluene diisocyanate has little effect on inflammatory mediator release from hPCLS. Supernatants from human PCLS exposed to salicylic acid (SA 10 uM, vehicle F-12 medium) or toluene diisocyanate (TDI 1 uM, vehicle 0.1% ethanol) for 24 h were screened using a custom Luminex cytokine/chemokine mediator array (Additional file 1: Table S1) to determine mediator release. SA or TDI had no significant effect on any of the 12 mediators analyzed by the array (data not shown). In additional ELISA assays, SA or TDI had little effect on a) IL-6, b) IL-8 or c) Eotaxin release from hPCLS, while the positive controls TNF-α (10 ng/ml) or IL-13 (100 ng/ml) increased IL-6, IL-8 and eotaxin levels, respectively. d) SA or TDI has little effect in tissue viability measured by LDH activity in culture supernatant (mean ± SEM of n = 4–5 donors; BL-baseline, Tri-1% Triton X-100). E) The toxicants had < 30% cytotoxicity at 0.1–100 μM (24 h) concentration range in HASM cells, measured by LDH activity in culture supernatants (Mean ± SD of n = 2 donors; BL-baseline)