Fig. 6

Influence of DNMT1 siRNA and 5-Aza on CSE-induced RASEF hypermethylation and transcription in HPASMCs. CSE reduced RASEF protein expression in HPASMCs (a), while 5-Aza (2 μM) increased them (b). c, d Pretreatment with either 5-Aza (2 μM) or DNMT1 siRNA inhibited CSE-induced downregulation of RASEF mRNA. e, f HPASMCs transfected with a DNMT1 overexpression vector reduced RASEF mRNA expression. g, i CSE-induced RASEF promoter CpG hypermethylation was prevented by pretreatment with either DNMT1 siRNA or 5-Aza (2 μM). Data are displayed as the mean ± standard error of the mean (n = 3); *P < 0.05, **P < 0.01