Fig. 2

Effect of cigarette smoke on DNMT1 levels in rat PAs and HPASMCs. a, b Western blot showed that cigarette smoke increased DNMT1 protein levels in rat PAs. c CCK8 assay revealed an increase in HPASMCs cell viability after treatment with different concentrations of CSE for 48 h. d, e DNMT1 protein expression in HPASMCs treated with CSE for 48 h. f Immunofluorescence results indicate an increase in DNMT1 protein levels (increased fluorescence) in CSE-treated HPASMCs. Data are displayed as the mean ± standard error of the mean (n = 3); (b) *P < 0.05 vs. the air group; (c, e) *P < 0.05, **P < 0.01, ***P < 0.001 vs. Con