Fig. 6

Increased TNF, IL-5 and IL-33 are observed in the lungs of dams fed a high fat diet, but not their offspring. From 4 weeks of age, female mice were fed either a low fat diet (LFD, n = 14) or high fat diet (HFD, n = 13). Female mice were mated with male mice, first after mice were fed either diet for 12 weeks, and secondly 1 week after the first litter of 2 week-old offspring were tested for lung function. The proportions of: (a) major cell types; (b) levels of TNF, IL-5, and IL-33; and, (c) adiponectin and leptin were measured in the BALF of offspring (2 week-old, n ≥ 5) and dams fed a LFD or HFD for 29 weeks. Data are shown as mean ± SEM (*p < 0.05), with some data points overlapping (particularly for part (A) dams)