Fig. 3

Female C57Bl/6 J mice fed a high fat diet developed metabolic dysfunction. From 4 weeks of age, female mice were fed either a low fat diet (LFD, n = 14) or high fat diet (HFD, n = 14). Female mice were mated with male mice, firstly after dams were fed either diet for 12 weeks, and secondly 1 week after the first litter of 2 week-old offspring were tested for lung function. In (a), fasting glucose at 5, 7, 9, 11 and 18 weeks after consuming either diet. In (b), fasting insulin at 7, 9, and 11 weeks after consuming either diet. In (c), results of glucose tolerance tests are shown for dams tested 11 weeks after consuming either diet, with glucose levels at various times post-injection of mice with glucose (1 g glucose/kg body weight), and (d), the area under the curve for these tests. Data are shown as mean ± SEM for n = 14 mice/treatment (*p < 0.05)