Fig. 9

Suppressive effect of a CCR2 antagonist on the expression of S100a8, S100a9, Slc26a4, and Il5 in Adgrf5^−/− lungs. Two-week-old Adgrf5^−/− mice were administered RS504393 (2 mg/kg body weight) or vehicle once daily via subcutaneous injection for 8 days. The mRNA expression of Ccl2, S100a8, S100a9, Saa3, Slc26a4, Clca1, Tgfb1, Il5, and Il13 was analyzed by qPCR using total RNA isolated from post-lavage lungs of injected mice and non-injected WT mice at 3 weeks of age. The data were normalized to Gapdh levels and are expressed as values relative to those from corresponding vehicle-treated Adgrf5^−/− mice. Values are presented as the mean ± SEM (n = 3–4). *p < 0.05; **p < 0.01; ***p < 0.001