Fig. 4
From: Galectin-3 enhances monocyte-derived macrophage efferocytosis of apoptotic granulocytes in asthma
Galectin-3 expression in MDMs. MDMs were obtained from blood taken from participants with asthma (n = 18) and healthy controls (n = 6). MDMs were transferred to cytospin slides, fixed and permeabilised with PLP. The cells were immunostained with a PE conjugated rat anti-human galectin-3 antibody (2.5 μg/mL) shown in red and the nuclei stained with DAPI in blue. (a-b) MDMs from the same healthy participant showing galectin-3 puncta (white arrows). (c) MDMs stained with a galectin-3-PE antibody from a different manufacturer also showing punctae. Scale bar, 10 μm (original magnification × 1000)