LncRNA H19 promotes the proliferation of pulmonary artery smooth muscle cells through AT1R via sponging let-7b in monocrotaline-induced pulmonary arterial hypertension

Su, Hua; Xu, Xiaoling; Yan, Chao; Shi, Yangfeng; Hu, Yanjie; Dong, Liangliang; Ying, Songmin; Ying, Kejing; Zhang, Ruifeng

doi:10.1186/s12931-018-0956-z

Table 1 Primers for RT-qPCR

From: LncRNA H19 promotes the proliferation of pulmonary artery smooth muscle cells through AT₁R via sponging let-7b in monocrotaline-induced pulmonary arterial hypertension

Gene	Accession Number	Species	Primer Pair Sequence 5′ to 3′
H19	NR_027324	Rat
Sense			GATGACAGGTGTGGTCAACG
Antisense			CAGACATGAGCTGGGTAGCA
H19	NR_130974	Mouse
Sense			CCACCCACTCACTCAGGATT
Antisense			GGGCAGAAGAGAACTCACCT
AT₁R	NM_030985	Rat
Sense			ACCAGGTCAAGTGGATTTCG
Antisense			ATCACCACCAAGCTGTTTCC
AT₁R	NM_175086	Mouse
Sense			GCAGCAGGGAGTAACAGAGA
Antisense			TGGGGCAGTCATCTTGGATT
β-actin	NM_031144	Rat
Sense			TGTCACCAACTGGGACGATA
Antisense			ACCCTCATAGATGGGCACAG
β-actin	NM_007393	Mouse
Sense			ACTGGGACGACATGGAGAAG
Antisense			ATGGGAGAACGGCAGAAGAA
PCNA	NM_022381	Rat
Sense			AGGACGGGGTGAAGTTTTCT
Antisense			CAGTGGAGTGGCTTTTGTGA
PCNA	NM_011045	Mouse
Sense			GAGAGCTTGGCAATGGGAAC
Antisense			ACTCTACAACAAGGGGCACA
Ki67	NM_139186	Rat
Sense			CATGGGGATTCTGAGGCTAA
Antisense			GGATCACTGCTTGCTCTTCC
Ki67	NM_001081117	Mouse
Sense			GCCATAACCCGAAAGAGCAG
Antisense			CCAGTTTACGCTTTGCAGGT
5sRNA	NR_033176	Rat	GTCTACGGCCATACCACCCTGAAC
5sRNA	NR_030686	Mouse	CTACAGCACCCGGTATTCCC
Let-7b	NR_031802	Rat	TGAGGTAGTAGGTTGTGTGGTT
Let-7b	NR_029727	Mouse	TGAGGTAGTAGGTTGTGTGGTT
universal r		Rat/Mouse	GCTGTCAACGATACGCTACGTAACG
Anchor RT		Rat/Mouse	GCTGTCAACGATACGCTACGTAACGGCATGACAGTG-ttttttttttttttttttttttttG
Let-7b mimic		Rat
Sense			UGAGGUAGUAGGUUGUGUGGUU
Antisense			AACCACACAACCUACUACCUCA
Mimic control		Rat
Sense			UUUGUACUACACAAAAGUACUG
Antisense			CAGUACUUUUGUGUAGUACAAA
Let-7b inhibitor		Rat	AACCACACAACCUACUACCUCA
Inhibitor control		Rat	UUUGUACUACACAAAAGUACUG
si-H19		Rat
Sense			CCUUUCCUGUAACUCUAUATT
Antisense			UAUAGAGUUACAGGAAAGGTT
si-AT₁R		Rat
Sense			GCAAUGUAUGUUUACCUCUAATT
Antisense			UUAGAGGUAAACAUACAUUGCTT
GAPDH	NM_017008	Rat
Sense			GAGACAGCCGCATCTTCTTG
Antisense			TGACTGTGCCGTTGAACTTG
GAPDH	NM_001289726	Mouse
Sense			CAACTCCCACTCTTCCACCT
Antisense			GAGTTGGGATAGGGCCTCTC

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ISSN: 1465-993X

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