Fig. 2

H19 interacts with miRNA let-7b. a: The diagram shows the structure of H19 luciferase in pGL3 reporter. The predicted binding sites for sponging let-7b and the corresponding deletion sites in the H19 sequence. The H19-Del represented the binding sites deletion (noted with a square red box). b: Dual luciferase activity assay. HEK-293 T cells were cotransfected with 200 ng of pGL3-H19 or pGL3-H19-Del with 20 ng of Renilla plasmid. A 40 nM miRNA control (miCon) or let-7b-mimic at a concentration of 10 nM, 20 nM and 40 nM was also added. c: PASMCs were transfected with 1000 ng of vector or pH 19 for 48 h. d: PASMCs were stimulated with PDGF-BB at a concentration of 100 ng/ml for 48 h. Then, PASMCs were transfected with 50 nM siRNA control (siCon) or siH19 for 24 h. e: Expression of let-7b in the lungs of MCT-induced rats and C57/BL6 mice (WT mice). H19 and let-7b were detected by RT-qPCR and normalized to β-actin and 5sRNA, respectively. Values were presented as means ± SD from 3 independent experiments. **0.001 ≤ P ≤ 0.009 (different from the corresponding control); ***P < 0.001 (different from the corresponding control)