Fig. 4

Changes in the number of inflammatory cells with fluid resuscitation. The number of circulating neutrophils decreased with endotoxemia before recovering post-saline resuscitation (a) with the NFR group recovering more quickly. Flow cytometry data revealed the levels of CD11b were upregulated on granulocytes in both groups, significantly decreasing post resuscitation in the NFR group (b). Neutrophil counts in BAL cytospins, as a percentage of total cells, were increased at 12 h with NFR (c). There was no change in the infiltration of macrophages in lung tissue with either group (d). Data are presented as mean ± SEM. n = 8 no fluid resuscitation, n = 8 fluid resuscitation. B = baseline; FR = fluid resuscitation; LPS = lipopolysaccharide; NFR = no fluid resuscitation; PMN = polymorphonuclear leukocyte. *p < 0.05, **p < 0.01, ***p < 0.001