Roles of mitochondrial ROS and NLRP3 inflammasome in multiple ozone-induced lung inflammation and emphysema

Respiratory Research

Table 1 The effects of MitoTEMPO and VX-765 on ozone-induced lung inflammation and emphysema

Parameter	MitoTEMPO	VX-765
IC, FRC, TLC, Cchord	NE	↓
FEV₂₅/FVC, FEV₅₀/FVC	NE	↑
Bronchoalveolar lavage cells	NE	↓total cells,macrophages, neutrophils,eosinophils
Lung inflammation score	↓	↓
Inflammatory cytokines	↓KC, IL-6	↓IL-1β, KC, IL-6
Oxidative stress	↓8-OHdG, lung mtROS	↓8-OHdG,lung mtROS
Mean linear intercept	NE	↓
Airway structure components	NE	↓Wam/Pbm, Wat/Pbm
Mitochondrial OXPHOS	↓complex II,IV	NE
Mitochondrial fission protein	↓DRP1,MFF	↓DRP1,MFF
Mitochondrial fusion protein	NE	NE
NLRP3 protein	↓	↓
Caspase-1 protein	NE	↓
Caspase-1 activity	NE	↓

The effects of MitoTEMPO and VX-765 on lung function,lung inflammation (bronchoalveolar lavage cells, inflammation score,Inflammatory cytokines), oxidative stress, lung histology(mean linear intercept, airway structure components), mitochondrial changes (OXPHOS, fission- and fusion-related proteins) and expression and activity of NLRP3/caspase-1
NE no effect ↓decrease ↑increase

ISSN: 1465-993X