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Fig. 7 | Respiratory Research

Fig. 7

From: Dietary ω-6 polyunsaturated fatty acid arachidonic acid increases inflammation, but inhibits ECM protein expression in COPD

Fig. 7

Reduced basal fibronectin and type I collagen expression upon arachidonic acid challenge. Pulmonary fibroblasts from COPD patients (n = 5) and non-COPD (n = 3–4) were unstimulated (control) or challenged with the ω-6 PUFA arachidonic acid (AA) in 0.1% BSA-DMEM (10 and 100 μM) for 48 h. Total RNA was collected and fibronectin (a), Type I collagen (1A2) (b) and tenascin (c) were detected using real time PCR array. The results are normalized to the endogenous control (18S rRNA), and presented as fold change from control ± standard error of the mean. Challenge with AA is compared to control using a Two-way ANOVA with LSD fisher’s test. Significance is represented as * (p < 0.05) and ** (p < 0.01)

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