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Fig. 3 | Respiratory Research

Fig. 3

From: Dietary ω-6 polyunsaturated fatty acid arachidonic acid increases inflammation, but inhibits ECM protein expression in COPD

Fig. 3

Arachidonic acid does not cause cytotoxicity in COPD and non-COPD fibroblasts. Pulmonary fibroblasts from COPD (n = 7) and non-COPD patients (n = 9) were unstimulated (control) or challenged with ω-6 PUFA arachidonic acid (AA) in 0.1% BSA-DMEM (100 μM) for 48 h. Cell free supernatants were collected and cell viability was estimated using lactate dehydrogenase (LDH) activity assay. Data is expressed as the absorbance (OD) at 490 nm ± standard error of the mean. Two-way ANOVA with Bonferroni post-hoc testing was used to determine statistical significance. There were no statistical differences

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