Table 1 Binding constants and thermodynamic parameters of actin-tropomyosin-myosin systems detected with ITC
From: cGMP interacts with tropomyosin and downregulates actin-tropomyosin-myosin complex interaction
Interaction | T | K(/mol) | ΔH (kcal/mol) | ΔS(kcal/mol) | ΔG(kcal/mol) |
|---|---|---|---|---|---|
Control/Actin+Myosin | 298.15 | 4.80 × 104 ± 1.22 × 105 | −30.70 ± 12.20 | −0.08 | − 30.70 ± 12.20 |
Tropomyosin/Actin+Myosin | 298.15 | 4.60 × 105 ± 7.84 × 104 | −5.83 × 105 ± 1.95 × 105 | −1.93 | − 5.82 × 105 ± 1.95 × 105 |
Tropomyosin/Actin+Myosin+ 8-Br-cGMP | 298.15 | 3.45 × 105 ± 4.34 × 104 | −2.49 × 107 ± 3.81 × 1011 | −8.34 × 104 | −3.43 × 104 ± 3.81 × 1011 |