Fig. 6

Analysis of actin-tropomyosin-myosin interactions by ITC. Raw titration data showed the thermal effect of protein interaction. (a) Diluted buffer was titrated into a calorimetric cell containing actin and myosin mixture. (b) Tropomyosin was titrated into a calorimetric cell containing actin and myosin mixture. (c) Tropomyosin mixtured with 8-Br-cGMP (100 μM) were titrated into a calorimetric cell containing actin and myosin mixture. The normalized heat of interaction was gained in all cases by integrating the raw data and subtracting the heat of ligand dilution into the buffer alone. The grey line represents the best fit obtained by a non-linear least-squares procedure based on an independent binding sites model. Experiments were repeated at least three times