Fig. 3
From: cGMP interacts with tropomyosin and downregulates actin-tropomyosin-myosin complex interaction
Identification of the association of cGMP and tropomyosin. (a) Proteins from Tropomyosin-Myc transfected HPASMC extracts were pulled down with the biotin-cGMP, separated in SDS-PAGE gels, and analyzed by immunoblotting with specific antibody to Myc. (b) Different amounts of biotin-cGMP were incubated with a mixture of protein extraction, and the eluted proteins were also subjected to SDS–PAGE. Antibodies against Myc were utilized in the Western blot analysis. (c) Various amounts of 8-Br-cGMP were added to compete with biotin-labeled cGMP in interacting with Tropomyosin-Myc