Fig. 1
From: cGMP interacts with tropomyosin and downregulates actin-tropomyosin-myosin complex interaction
cGMP directly interacts with tropomyosin. (a) The chemical structural formula of 8-[Biotin]-AET-cGMP. Biotin is connected to the 8-position of cyclic GMP via a 11 atom spacer. (b) Characterization of interaction proteins with cGMP in vitro. Cell extractions from HPASMCs were pulled down with the biotin-cGMP, subjected to SDS–PAGE and visualized by silver staining. The bands associated with cGMP were pointed out by the numbers (bands 1–6) and identified by mass spectrometry. The peptides of the band 1 belong to tropomyosin are indicated by red letters