A circulating cell population showing both M1 and M2 monocyte/macrophage surface markers characterizes systemic sclerosis patients with lung involvement

Trombetta, Amelia Chiara; Soldano, Stefano; Contini, Paola; Tomatis, Veronica; Ruaro, Barbara; Paolino, Sabrina; Brizzolara, Renata; Montagna, Paola; Sulli, Alberto; Pizzorni, Carmen; Smith, Vanessa; Cutolo, Maurizio

doi:10.1186/s12931-018-0891-z

Table 2 The CD204 positive cell population percentages are shown in patients with (SSc-ILD) or without interstitial lung disease (SSc-No ILD) at lung CT scan and healthy subjects (HSs)

From: A circulating cell population showing both M1 and M2 monocyte/macrophage surface markers characterizes systemic sclerosis patients with lung involvement

Analysis of circulating CD204+ cells	SSc-ILD (37)	SSc-No-ILD (18)	p (MW)	HSs (27)	p (KW)
CD204⁺ (%)	0.5 ± 0.40	0.8 ± 0.7	p = 0.13	0.7 ± 0.3	0.21
CD204⁺CD163⁺ (%leukocytes)	0.08 ± 0.22	0.09 ± 0.14	p = 0.65	0.03 ± 0.03	0.001
CD204⁺CD163⁺ (%CD204⁺)	13.7 ± 15	8.4 ± 13	p = 0.034	6.3 ± 3	< 0.0001
CD204⁺CD163⁺TLR4⁺ (%leukocytes)	0.03 ± 0.22	0.02 ± 0.15	p = 0.34	0.008 ± 0.01	< 0.0001
CD204⁺CD163⁺TLR4⁺ (%CD204⁺)	6.2 ± 16	2.9 ± 15	p = 0.025	1.4 ± 1.7	< 0.0001
CD204⁺CD163⁺CD206⁺ (%leukocytes)	0.01 ± 0.1	0.01 ± 0.05	p = 0.65	0.008 ± 0.01	0.001
CD204⁺CD163⁺CD206⁺ (%CD204⁺)	4 ± 7.4	1.9 ± 5.6	p = 0.07	1.1 ± 1.2	< 0.0001
CD204⁺CD163⁺CD206⁺TLR4⁺ (%leukocytes)	0.014 ± 0.1	0.11 ± 0.05	p = 0.20	0.003 ± 0.004	< 0.0001
CD204⁺CD163⁺CD206⁺TLR4⁺ (%CD204⁺)	2.7 ± 7.3	1.1 ± 5.9	p = 0.013	0.5 ± 0.6	< 0.0001
CD204⁺CD163⁺CD206⁺ TLR4⁺CD14⁺(%leukocytes)	0.003 ± 0.014	0.004 ± 0.003	p = 0.795	0.001 ± 0.003	0.008
CD204⁺CD163⁺CD206⁺ TLR4⁺CD14⁺(%CD204⁺)	0.73 ± 1.4	0.27 ± 0.54	p = 0.097	0.20 ± 0.38	< 0.0001
CD204⁺CD163⁺CD206⁺ TLR4⁺CD14⁻ (%leukocytes)	0.009 ± 0.08	0.006 ± 0.05	p = 0.092	0.001 ± 0.002	< 0.0001
CD204⁺CD163⁺CD206⁺ TLR4⁺CD14⁻ (%CD204+)	1.93 ± 6.56	0.6 ± 5.69	p = 0.029	0.17 ± 0.41	< 0.0001
CD204⁺CD163⁺CD206⁺ TLR4⁺CD80⁺ (%leukocytes)	0.01 ± 0.09	0.004 ± 0.05	p = 0.041	0.001 ± 0.003	< 0.0001
CD204⁺CD163⁺CD206⁺ TLR4⁺CD80⁺ (%CD204⁺)	2.07 ± 6.83	0.5 ± 5.33	p = 0.010	0.16 ± 0.48	< 0.0001
CD204⁺CD163⁺CD206⁺ TLR4⁺CD86⁺ (%leukocytes)	0.008 ± 0.08	0.005 ± 0.04	p = 0.082	0.001 ± 0.002	< 0.0001
CD204⁺CD163⁺CD206⁺TLR4⁺ CD86⁺ (%CD204⁺)	1.16 ± 5.8	0.72 ± 4.1	p = 0.023	0.19 ± 0.36	< 0.0001
CD204⁺CD163⁺CD206⁺TLR4⁺ CD80⁺CD86⁺ (%leukocytes)	0.04 ± 0.08	0.002 ± 0.03	p = 0.036	0.0006 ± 0.001	< 0.0001
CD204⁺CD163⁺CD206⁺TLR4⁺ CD80⁺CD86⁺ (%CD204⁺)	1 ± 5.6	0.39 ± 4	p = 0.021	0.08 ± 0.2	< 0.0001

By Mann-Whitney test, several mixed M1/M2 cell populations were found to show significantly higher percentages (p MW highlighted in bold) in SSc patients affected by ILD, compared to SSc patients with no ILD. On the right, Kruskal-Wallis test was performed adding HSs data, obtaining more significant results (p KW)

Back to article page

ISSN: 1465-993X

Contact us

General enquiries: journalsubmissions@springernature.com

Respiratory Research

Contact us