Fig. 2From: Differential effects of Nintedanib and Pirfenidone on lung alveolar epithelial cell function in ex vivo murine and human lung tissue cultures of pulmonary fibrosisEffect of ex vivo treatment with Pirfenidone and Nintedanib on lung epithelial cell marker in fibrotic 3D-LTCs. a-c Fibrotic 3D-LTCs were cultured for 48 h in the presence of anti-fibrotic drugs Nintedanib (1 μM) and Pirfenidone (500 μM). a proSP-C expression was assessed by Western blot. β-Actin was used as loading control. Quantification of proSP-C Western blot, n = 6. Data was normalized to β-Actin. b, c SP-C and WISP1 secretion of 3D-LTCs was determined by ELISA. n = 4–7. Significance was assessed using Wilcoxon matched pairs test. Significance: *p < 0.05Back to article page