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Fig. 2 | Respiratory Research

Fig. 2

From: Differential effects of Nintedanib and Pirfenidone on lung alveolar epithelial cell function in ex vivo murine and human lung tissue cultures of pulmonary fibrosis

Fig. 2

Effect of ex vivo treatment with Pirfenidone and Nintedanib on lung epithelial cell marker in fibrotic 3D-LTCs. a-c Fibrotic 3D-LTCs were cultured for 48 h in the presence of anti-fibrotic drugs Nintedanib (1 μM) and Pirfenidone (500 μM). a proSP-C expression was assessed by Western blot. β-Actin was used as loading control. Quantification of proSP-C Western blot, n = 6. Data was normalized to β-Actin. b, c SP-C and WISP1 secretion of 3D-LTCs was determined by ELISA. n = 4–7. Significance was assessed using Wilcoxon matched pairs test. Significance: *p < 0.05

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