Fig. 6
From: Elastin degradation products in acute lung injury induced by gastric contents aspiration
In vitro tropoelastin degradation pattern is not dependent on acidity but it is associated with the presence of elastase. Representative Western blot of soluble elastin illustrating the effect of acid and elastase on the pattern of elastin fragmentation. Lane 1 corresponds to lung tissue homogenate from a control rat showing only the 70 kDa band, likely corresponding to tropoelastin. Lanes 2 to 5 correspond to lung tissue homogenate from a control rat treated with hydrochloric acid at 37 °C to a final pH:1.6, for 2, 4, 6 and 8 h, showing the 70 kDa band, without evidence of smaller molecular weight elastin-derived peptides. Lane 6 corresponds to lung tissue homogenate from a control rat treated with commercially available porcine pancreatic elastase showing the 70 kDa band and a pattern of fragmentation similar to that seen in lane 7, corresponding to lung tissue homogenate from a rat studied 12 h after gastric fluid instillation. β-tubulin immunoblot shows protein loading. h: hours