Fig. 5
From: IL-32γ attenuates airway fibrosis by modulating the integrin-FAK signaling pathway in fibroblasts
rIL-32γ inhibits integrin-mediated activation of FAK/paxillin. Phosphorylation of FAK and paxillin was detected in TGF-β (5 ng/mL)-stimulated MRC-5 cells pretreated with an RGD peptide (a) or rIL-32γ (b). Activated FAK and paxillin were detected after 24 h. Results are representative of three independent experiments. c MRC-5 cells were stimulated with TGF-β for 24 h, and His-tagged rIL-32γ was precipitated from cell lysates using Ni-NTA beads. Bound proteins were analyzed by Western blotting with antibodies specific for integrin β3, paxillin, and the His-tag. d Flag-tagged IL-32γ-overexpressing MRC-5 cells were stimulated with TGF-β and harvested at 24 h. Flag-tagged IL-32γ was then immunoprecipitated from cell lysates using an anti-flag antibody followed by immunoblotting with an anti-FAK antibody. Similar results were obtained from two independent experiments. FT, flow-through; W, wash; E, elution