Fig. 5

Mean ± SD proportions (percent of mono-sulphated and non-sulphated [CS] disaccharides) of the mono-sulphated (∆-di-4S and ∆-di-6S) and unsulphated (∆-di-0S) CS disaccharides measured in micro-dissected fetal lung tissue from fetuses (a) exposed to cortisol-infusion (131d n = 6), (c) exposed to antenatal betamethasone treatment (Beta, 124d, n = 6) or (e) bilaterally adrenalectomised (ADX, 143d, n = 6) and their relevant control fetuses. All values were measured by FACE analysis FACE gels depicting the sulphation profile of CS Δ-disaccharides in the lungs of fetuses (b) exposed to cortisol-infusion, (d) exposed to antenatal betamethasone treatment or (f) bilaterally adrenalectomised and their relevant control fetuses. A control sample without chondroitinase ABC digestion (labelled undigested) was electrophoresed alongside each chondroitinase ABC-digested sample in order to identify non-specific bands (arrow-heads). The density of non-specific background bands (migrating at the same position as specific bands) were substracted from the density of the bands of interest