Fig. 3

Effect of ectopic miR-22 mimic on CD147 and HDAC4 in pBECs of non-asthmatics and asthmatics. pBECs cultured as monolayers were transfected with miR-22 mimic (5 nM) for 24 or 48 h. a-b Transfection was confirmed by assessment of miR-22 expression, and the miR-22 mimic increased miR-22 expression after 24 h in both groups. c-f CD147 mRNA expression was reduced after 24 h in asthmatics and protein expression was suppressed after 48 h in both group. g-j HDAC4 mRNA expression was reduced in asthmatics after 24 h and protein expression was suppressed after 48 h in non-asthmatics. *P ≤ 0.05, using Mann-Whitney nonparametric test, N ≥ 3