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Table 6 Sensitivity Analyses of Association between FEV1/FVC and Methylation at cg02181506, cg2462102, and cg08257009, SAPALDIA Cohort

From: SERPINA1 methylation and lung function in tobacco-smoke exposed European children and adults: a meta-analysis of ALEC population-based cohorts

  cg02181506 cg24621042 cg08257009
Coef. P value Coef. P value Coef. P value
FEV1/FVC repeat cross-sectional
 Main modela −0.01 0.93 0.05 0.54 0.07 0.12
 Adjustment for PiS and PiZ genotype −0.01 0.94 0.05 0.54 0.07 0.12
 Adjustment for neutrophils at T1 and T2 −0.00 0.97 0.05 0.52 0.07 0.11
 Men 0.03 0.86 0.07 0.47 0.10 0.11
 Women −0.07 0.65 0.06 0.62 0.05 0.49
 Not obese (BMI < 30 kg/m2) −0.06 0.58 0.04 0.60 0.12 0.01
 Obese (BMI ≥ 30 kg/m2) 0.19 0.25 0.06 0.68 −0.12 0.14
 No self-report of ever asthma at T1 and T2 −0.03 0.78 0.07 0.35 0.08 0.09
 Asthma ever self-report at T1 or T2 0.14 0.44 −0.05 0.72 −0.01 0.88
FEV1/FVC changeb
 Main modelc −0.01 0.60 −0.01 0.49 0.02 0.005
 Adjustment for circulating hs-crp and AATd −0.008 0.56 −0.007 0.01 0.02 0.006
 Adjustment for PiS and PiZ genotype −0.01 0.57 −0.01 0.48 0.02 0.005
 Adjustment for neutrophils at T1 and T2 −0.01 0.44 −0.01 0.36 0.02 0.01
 Men 0.01 0.77 −0.00 0.74 0.02 0.01
 Women −0.03 0.14 −0.01 0.56 0.02 0.08
 Not obese (BMI < 30 kg/m2) at T1 −0.00 0.84 −0.00 0.87 0.02 0.01
 Obese (BMI ≥ 30 kg/m2) at T1 −0.04 0.13 −0.04 0.07 0.02 0.17
 No self-report of ever asthma at T1 and T2 −0.01 0.57 −0.01 0.40 0.01 0.04
 Asthma ever self-report at T1 or T2 −0.03 0.28 0.01 0.65 0.03 0.04
  1. aAdjusted for: study center, age, age2, education, height, height2, sex, sex*age, (sex*age)2, sex*height, (sex*height)2, Bcell, CD4T, CD8T, Eos, Mono, NK and ran with a random intercept on the subject
  2. bDirection of effect. A positive sign indicates that an increase in methylation is associated with higher level of lung function (cross-sectional models) and with an attenuation of lung function decline in the prediction models (change in lung function)
  3. cAdjusted for covariates at T1: study center, age, age2, education, height, height2, sex, sex*age, (sex*age)2, sex*height, (sex*height)2, Bcell, CD4T, CD8T, Eos, Mono, NK. Change in lung function was computed as the difference between T2 and T1 divided by time of follow-up: (T2-T1)/follow-up
  4. dCirculating hs-crp and AAT available from T1 only
  5. Statistically significant results are highlighted in bold