SERPINA1 methylation and lung function in tobacco-smoke exposed European children and adults: a meta-analysis of ALEC population-based cohorts

Table 6 Sensitivity Analyses of Association between FEV₁/FVC and Methylation at cg02181506, cg2462102, and cg08257009, SAPALDIA Cohort

	cg02181506		cg24621042		cg08257009
	Coef.	P _value	Coef.	P _value	Coef.	P _value
FEV₁/FVC repeat cross-sectional
Main model^a	−0.01	0.93	0.05	0.54	0.07	0.12
Adjustment for PiS and PiZ genotype	−0.01	0.94	0.05	0.54	0.07	0.12
Adjustment for neutrophils at T1 and T2	−0.00	0.97	0.05	0.52	0.07	0.11
Men	0.03	0.86	0.07	0.47	0.10	0.11
Women	−0.07	0.65	0.06	0.62	0.05	0.49
Not obese (BMI < 30 kg/m²)	−0.06	0.58	0.04	0.60	0.12	0.01
Obese (BMI ≥ 30 kg/m²)	0.19	0.25	0.06	0.68	−0.12	0.14
No self-report of ever asthma at T1 and T2	−0.03	0.78	0.07	0.35	0.08	0.09
Asthma ever self-report at T1 or T2	0.14	0.44	−0.05	0.72	−0.01	0.88
FEV₁/FVC change^b
Main model^c	−0.01	0.60	−0.01	0.49	0.02	0.005
Adjustment for circulating hs-crp and AAT^d	−0.008	0.56	−0.007	0.01	0.02	0.006
Adjustment for PiS and PiZ genotype	−0.01	0.57	−0.01	0.48	0.02	0.005
Adjustment for neutrophils at T1 and T2	−0.01	0.44	−0.01	0.36	0.02	0.01
Men	0.01	0.77	−0.00	0.74	0.02	0.01
Women	−0.03	0.14	−0.01	0.56	0.02	0.08
Not obese (BMI < 30 kg/m²) at T1	−0.00	0.84	−0.00	0.87	0.02	0.01
Obese (BMI ≥ 30 kg/m²) at T1	−0.04	0.13	−0.04	0.07	0.02	0.17
No self-report of ever asthma at T1 and T2	−0.01	0.57	−0.01	0.40	0.01	0.04
Asthma ever self-report at T1 or T2	−0.03	0.28	0.01	0.65	0.03	0.04

^aAdjusted for: study center, age, age², education, height, height², sex, sex*age, (sex*age)², sex*height, (sex*height)², Bcell, CD4T, CD8T, Eos, Mono, NK and ran with a random intercept on the subject
^bDirection of effect. A positive sign indicates that an increase in methylation is associated with higher level of lung function (cross-sectional models) and with an attenuation of lung function decline in the prediction models (change in lung function)
^cAdjusted for covariates at T1: study center, age, age², education, height, height², sex, sex*age, (sex*age)², sex*height, (sex*height)², Bcell, CD4T, CD8T, Eos, Mono, NK. Change in lung function was computed as the difference between T2 and T1 divided by time of follow-up: (T2-T1)/follow-up
^dCirculating hs-crp and AAT available from T1 only
Statistically significant results are highlighted in bold

ISSN: 1465-993X