Fig. 2
From: Impact of immunization against OxLDL on the pulmonary response to cigarette smoke exposure in mice
OxLDL immunization does not exacerbate the pulmonary inflammatory response to cigarette smoke exposure. Six to eight weeks old female BALB/c mice (n = 5/group) were injected with PBS alone or immunized against OxLDL (OxLDL:Addavax) and exposed to room air or cigarette smoke for 2 (a-c) or 8 weeks (d-f). Bronchoalveolar lavage (BAL) total cell numbers and differential cell (mononuclear cells and neutrophils) counts were assessed in (a) the 2-week and (d) the 8-week exposure protocols. BAL fluid MCP-1 protein levels and lung cxcl5 mRNA levels were also measured in (b-c) the 2-week and (e-f) the 8-week exposure protocols. g Representative cytospins from total BAL cells stained with haematoxylin and eosin. One-way ANOVA followed by Tukey’s multiple comparison tests *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; NS = not significantly different after two-sided Student T test