Table 2 Pair wise comparison of BM and circulating inflammatory and repair markers (median [IQR] or mean ± SD) in the three groups studied. Bolded italic text highlights variables with statistically significant differences (p ≤ 0.05)
From: Bone marrow characterization in COPD: a multi-level network analysis
Never-smokers (n = 15) | Smokers (n = 10) | COPD (n = 35) | ANOVA (or KW) P Value | |
|---|---|---|---|---|
BM CHARACTERIZATION | ||||
Immunophenotype | ||||
CD3% | 5.00 [5.00–6.00] | 5.50 [3.30–7.00] | 6.00 [4.00–9.50] | 0.59 |
CD5% | 5.00 [5.00–6.00] | 5.00 [3.00–7.00] | 6.00 [4.30–9.80] | 0.40 |
CD7% | 7.00 [5.30–8.00] | 6.00 [4.00–9.00] | 6.00 [5.00–9.80] | 0.78 |
CD10% | 20.00 [13.00–26.00] | 19.00 [15.00–28.50] | 19.50 [15.80–25.80] | 0.79 |
CD19 + % | 3.00 [2.00–4.50] | 3.00 [2.00–6.30] | 3.00 [2.00–4.00] | 0.57 |
CD19 + CD10 + % | 2.00 [1.30–3.00] | 0.50[0.50–2.50] | 2.00 [1.00–2.00] | 0.62 |
CD33% | 76.00 [68.50–80.00] | 68.50 [61.30–79.00] | 71.50 [65.00–80.00] | 0.47 |
CD13% | 34.50 [30.00–39.50] | 30.00 [20.00–40.00] | 30.00 [25.00–33.00] | 0.43 |
CD14% | 2.00 [1.30–3.80] | 3.00 [2.00–4.00] | 3.00 [2.00–4.00] | 0.31 |
CD15% | 70.00 [57.50–75.00] | 62.50 [56.30–73.80] | 65.00 [60.00–70.00] | 0.70 |
CD117% | 2.00 [1.50–2.00] | 2.00 [2.00–2.80] | 2.00 [2.00–2.80] | 0.40 |
CD34% | 1.00 [1.00–2.00] | 2.00 [1.00–2.80] | 1.00 [1.00–2.00] | 0.50 |
DR% | 8.00 [4.50–11.00] | 9.50 [7.30–10.00] | 8.00 [7.503–10.00] | 0.81 |
CD4% | 3.00 [3.00–3.00] | 3.00 [2.00–3.40] | 3.50 [3.00–4.80] | 0.24 |
CD8% | 2.00 [2.00–3.00] | 2.00 [1.30–4.00] | 3.00 [2.00–4.00] | 0.66 |
CD56% | 1.00 [1.00–1.80] | 1.00 [1.00–2.00] | 1.00 [1.00–1.00] | 0.83 |
sCD64% | 3.00 [1.50–5.00] | 3.00 [2.00–4.00] | 3.00 [2.00–4.00] | 0.88 |
Progenitor cell surface markers | ||||
CD34 | ||||
% | 7.38 [2.28–16.23] | 13.44 [10.50–18.02] | 14.50 [9.83–24.10] | 0.07 |
MFI | 164.60 [110.50–202.80] | 118.03 [54.99–335.73] | 92.65 [65.85–113.10] | 0.09 |
CD 34 + c-kit+ | ||||
% | 76.40 [67.00–85.40] | 65.98 [57.88–79.71] | 81.90 [66.80–84.70] | 0.41 |
MFI | 24.70 [19.60–39.65] | 19.95 [15.85–70.73] | 38.10 [24.80–55.40] | 0.36 |
CD 34 + Ki67+ | ||||
% | 69.40 [30.30–77.40] | 83.30 [59.99–97.64] | 96.80 [73.30–97.90] | 0.72 |
MFI | 31.80 [22.35–52.90] | 28.00 [21.75–70.65] | 50.30 [43.30–74.30] | 0.32 |
CD34 + 133 + KDR+ | ||||
% | 1.10 [0.00–3.05] | 1.23 [0.18–1.80] | 4.35 [1.58–7.73] | 0.51 |
MFI | 78.00 [37.93–127.15] | 112.40 [74.48–134.73] | 49.25 [40.10–120.60] | 0.33 |
INFLAMMATORY AND REPAIR MARKERS | ||||
BM Supernatant | ||||
IL6 pg/ml | 12.20 [1.30–23.00] | 13.06 [1.65–27.39] | 9.80 [7.60–24.90] | 0.06 |
IL-8 pg/ml | 6.50 [3.90–10.70] | 7.10 [3.00–8.70] | 3.60 [2.40–10-90] | 0.48 |
HGF pg/ml | 2854.07 [646.71–22,718.86] | 1290.97 [909.37–9284.43] | 1689.59 [692.43–2466.46] | 0.63 |
Circulating blood | ||||
Leucocytes ×10^9/L | 7.67 ± 3.43 | 8.41 ± 2.45 | 7.41 ± 1.88 | 0.52 |
Neutrophils % | 55.07 ± 18.58 * | 71.98 ± 11.92 | 61.69 ± 11.85 | 0.02 |
Eos % | 2.13 ± 1.77 | 1.46 ± 1.19 | 3.14 ± 1.65 † | 0.01 |
CRP mg/dl | 1.62 ± 2.05 | 0.90 ± 2.19 | 5.65 ± 12.57 ‡ | 0.02 |
IL-6 pg/ml | 0.10 [0.10–0.10] | 0.10 [0.00–0.10] | 0.10 [0.1–0.20] | 0.14 |
IL-8 pg/ml | 1.40 [0.90–2.50] | 2.80 [1.30–5.90] | 2.30 [1.5–3.10] | 0.82 |