Fig. 2

The representative gating strategy for assessing myeloid cell sub-populations in the lung. The population hierarchy shows the gating strategy (a). ‘Single’ cells (excluding doublets and triplets) were selected with a polygon gate on a FSC-A vs. FSC-H dot plot (b). Single cells were gated on the resulting daughter population on a FSC-A vs. SCA-A dot plot (b). A ‘Live’ cell gate (which excludes debris) was created with the aid of the CD45⁺ cells on FSC-A vs. CD45⁺ (b). CD45⁺CD11b⁺CD11c⁺ alveolar macrophages were selected with a gate (b), CD45⁺ F4/80⁺ CD206⁺ M1 vs. M2 macrophages were selected with a gate (d) and CD45⁺Ly6C⁺Ly6G⁺ granulocytes (e) were selected for further analysis of myeloid cell subsets. Plots in c, d and e are from a hyperoxic lung taken at day 0, day 4, and day 7 from left to right