Fig. 5

Effect of glucocorticoid treatment on CS co-exposure mouse models of allergic asthma. Sensitised male mice were challenged with intranasal saline (50 ul) or antigen (OVA or HDM) once a day for 3 days. Mice were co-exposed to room air or CS for eight days. BALF was then collected and the numbers of white cells assessed. Mice were dosed with vehicle (10 ml/kg, orally, p.o.) or budesonide (3 mg/kg, p.o.) one hour prior to the morning CS challenges and one hour after afternoon CS challenges. Data are represented as mean ± S.E.M. for n = 8 animals in each group. a: Eosinophil number, b: Neutrophil number and c: lymphocyte number. The statistical significance of the response to antigen and/or CS was determined using a Mann-Whitney U test and denoted with * (P < 0.05). The significance of the impact of budesonide was determined using one way ANOVA followed by a Bonferoni’s correction post-test # (P < 0.05)