Fig. 1
Effect of the hydrogen sulfide (H2S) donors, sodium hydrogen sulfide (NaSH) and GYY4137, on airway smooth muscle (ASM) from non-smokers, smokers and COPD patients. ASM cells were incubated with FCS (2.5%) for 1 h; NaSH (100 μM) or GYY4137 (100 μM) was added for another 8 days. Cellular growth and survival (a), DNA synthesis (b), IL-6 release (c), and CXCL8 release (d) were subsequently measured by dimethylthiazol-diphenyltetrazolium bromide (MTT) assay, bromodeoxyuridine (BrdU) ELISA, and DuoSet ELISA respectively. Bars represent means (± SEM) of nine ASM donors. */+/#P < 0.05; **/++/##P < 0.01; ***/+++/###P < 0.001