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Fig. 6 | Respiratory Research

Fig. 6

From: Resolution of bleomycin-induced murine pulmonary fibrosis via a splenic lymphocyte subpopulation

Fig. 6

Effect of adoptively transferring Tregs isolated from interleukin (IL)-10 knock-out mice on pulmonary fibrosis. Tregs isolated from wild-type and IL-10 knock-out mice were injected via the tail vein (1 × 106/mouse) on day 14 after initiating BLM treatment. On day 28 post-BLM challenge, the mice were sacrificed and their lungs were removed for analyses. af Adoptive transfer of Tregs isolated from IL-10 knock-out mice did not attenuate fibrosis and collagen deposition induced by BLM treatment. Representative photomicrographs following hematoxylin and eosin (HE) and Masson’s trichrome staining are presented. Magnification × 40. g The extent of lung fibrosis was measured on day 28 by quantitative histology following Ashcroft’s method to determine the anti-fibrotic effects of Tregs obtained from both wild-type and IL-10 knock-out mice. Adoptive transfer of Tregs isolated from IL-10 knock-out mice did not ameliorate the lesion. Data are displayed as median and interquartile range. h The hydroxyproline content in the lungs was measured on day 28. A similar trend for the numerical score was observed. n = 4 mice/group. WT; wild-type, IL-10−/−; IL-10 knock-out

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