Fig. 3

Neither FAK, Src, nor ERK1/2 activation is affected by FKBP10 kd. Western Blot analysis of phLF treated with scrambled siRNA as control (sc) or FKBP10 siRNA (kd) and 2- phosphoascorbate (0.1 mM) in absence and presence of TGF-β1 (2 ng/mL) for 48h. Densitometric analysis and representative blots show the effect of FKBP10 kd on the expression of FAK (a), Src (d) and ERK1 (f) relative to β-actin as loading control (ACTB). b, c Densitometric analysis and representative blots showing the effect of FKBP10 kd on the phosphorylation of FAK (Y397) (b) and FAK (Y576/577) (c) relative to FAK. e, g Densitometric analysis and representative blots showing the effect of FKBP10 kd on the phosphorylation of Src (Y416) (e) relative to Src and pERK1/2 (T202/Y204) (g) relative to ERK1. All data is based on eight independent experiments. Statistical significance between control and FKBP10 kd is indicated by horizontal brackets and asterisks