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Fig. 3 | Respiratory Research

Fig. 3

From: Pulmonary inflammation-induced loss and subsequent recovery of skeletal muscle mass require functional poly-ubiquitin conjugation

Fig. 3

Changes in protein synthesis signaling correspond to muscle mass loss and recovery following pulmonary inflammation. UBWT and K48R mice were subjected to the intratracheal instillation of NaCl or LPS, and gastrocnemius muscle was collected at the indicated time points (hours after instillation). Protein levels of phosphorylated p70S6 (Thr389), total p70S6, phosphorylated S6 (ser235/236), total S6, phosphorylated 4EBP1 (ser65 and Thr37/46) and total 4EBP1 were assessed in lysates of gastrocnemius muscle tissue with western blot analysis. (e) Representative western blots of the indicated proteins. Ratios of (a) phosphorylated p70S6 over total p70S6, (b) phosphorylated S6 over total S6, (c) phosphorylated 4EBP1 (ser65) over total 4EBP1 and (d) phosphorylated 4EBP1 (Thr37/46) over total 4EBP1. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with control (intratracheal NaCl), * above a line refers to a difference in response between genotypes

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