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Fig. 2 | Respiratory Research

Fig. 2

From: Pulmonary inflammation-induced loss and subsequent recovery of skeletal muscle mass require functional poly-ubiquitin conjugation

Fig. 2

Transient activation of autophagy during muscle atrophy following pulmonary inflammation. UBWT and K48R mice were subjected to the intratracheal instillation of NaCl or LPS, and gastrocnemius muscle was collected at the indicated time points (hours after instillation). Protein levels of (b) LC3B-I and LC3B-II, (d, e) p62, and (h) phosphorylated ULK1 (ser757) and total ULK1 were assessed in lysates of gastrocnemius muscle tissue with western blot analysis. a) Ratio of LC3B-II over LC3B-I. g) Ratio of phosphorylated ULK1 over total ULK1. mRNA transcript levels of (c) LC3B and (f) p62 were determined, normalized to geNorm, and expressed as fold change compared with UBWT intratracheal NaCl. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with control (intratracheal NaCl), * above a line refers to a difference in response between genotypes

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