Fig. 7
c-Abl phosphorylation is regulated by Src and actin polymerization. a Mouse tracheal rings pretreated with the Src inhibitor PP2 (10 μM, 30 min) were stimulated with ACh or unstimulated. c-Abl phosphorylation was evaluated by immunoblotting. Data are mean ± SE (n = 4). b Treatment with PP2 attenuates contractile response of tracheal segments to ACh (n = 4–6). Contractile force is normalized to the force induced by 10−5 M ACh before addition of PP2. c Immunoblotting was used to evaluate c-Abl phosphorylation of unstimulated and stimulated mouse tracheal rings pretreated with the actin polymerization inhibitor latrunculin A (1 μM, 30 min). (n = 4). d Contraction of mouse tracheal rings were reduced by treatment with latrunculin A (Lat-A). Contractile force is normalized to the force induced by 10−5 M ACh before addition of Lat-A. **P < 0.01; NS, not significant