Fig. 4

Treatment with CAS peptide attenuates CAS/Pfn-1 coupling and spatial redistribution of CAS and Pfn-1 upon contractile stimulation. a Mouse tracheal rings were pretreated with control (Con) or CAS peptide (2.5 μg/ml) for 30 min. They were then stimulated with ACh (100 μM, 5 min), or left untreated (UT). The protein-protein interaction was evaluated by co-immunoprecipitation. Data are mean ± SE (n = 4). b HASM cells pretreated with control or CAS peptide were stimulated with ACh (100 μM, 5 min), or were untreated. The spatial localization of CAS and Pfn-1 in the cells was assessed by immunostaining. Arrows indicate a single line scan to analyze the fluorescent intensity for each cell. The inset plots represent fluorescent intensity of the line scan indicated by the arrows. c Fluorescent ratios of cell periphery over interior for CAS or Pfn-1 in cells were calculated. Data are mean ± SE (n = 26 cells from 4 independent experiments). **P < 0.01. UT, untreated