Fig. 3
From: PM2.5 promotes human bronchial smooth muscle cell migration via the sonic hedgehog signaling pathway
Effects of PM2.5 on the Shh pathway in HBSMCs. a TAPM2.5 and (b) WSPM2.5 increased Shh and Gli1 expression in HBSMCs in a time-dependent manner. c TAPM2.5 and (d) WSPM2.5 increased Snail expression in HBSMCs in a time-dependent manner. e and f Cyclopamine significantly attenuated the effects of TAPM2.5 and WSPM2.5 on Gli1 expression, but had no effect on Shh expression. g and h Cyclopamine significantly attenuated the effects of TAPM2.5 and WSPM2.5 on Snail expression. i and j siRNA knockdown of Gli1 significantly attenuated the effects of TAPM2.5 and WSPM2.5 on Gli1 expression. k and l siRNA knockdown of Gli1 significantly attenuated the effects of TAPM2.5 and WSPM2.5 on Snail expression. *, P < 0.05; **, P < 0.001; #, P < 0.05; ##, P < 0.001 compared with the control group