Fig. 2

Bronchoalveolar lavage (BAL) total (a) and differential cell counts (b, c) for wildtype (WT) and mice not expressing TLR4 (TLR4−/−) for each treatment. Elevated total cell counts in BAL were observed in WT mice treated with AMP, LPS and AMP-LPS compared to WT control mice. Cell counts in WT mice treated with LPS and AMP-LPS were also greater than similarly treated TLR4−/− mice. Neutrophils were the predominant cell type in LPS and AMP-LPS treated WT mice. Macrophages dominated counts in AMP treated WT and TLR4−/− mice, as well as LPS and AMP-LPS treated TLR4−/− mice compared to respective controls. Greater eosinophil numbers were observed in WT mice treated with LPS and AMP-LPS; for which numbers were greater in AMP-LPS treated mice. Lymphocyte and epithelial cell numbers were not significantly different between controls and any of the treatments given for either strain (*p < 0.05 between treatment and control; # = p < 0.05 between strains for the same treatment; α = p < 0.05 between treatment compared to all other treatments for the same strain)