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Fig. 5 | Respiratory Research

Fig. 5

From: RNA-sequencing analysis of lung primary fibroblast response to eosinophil-degranulation products predicts downstream effects on inflammation, tissue remodeling and lipid metabolism

Fig. 5

PCR analysis demonstrates upregulation of CXCL1, CXCL8, IL6 and ICAM1 expression levels in HLF activated with conditioned media from eosinophils pre-activated with IL3 and degranulating on aggregated IgG. Conditioned media were prepared from eosinophils pre-activated for 20 h with IL-3 or IL-5, and seeded on heat-aggregated human IgG for 6 h (IL3IgG or IL5IgG). Conditioned media from eosinophils pre-activated with IL-3 and seeded in uncoated wells (IL3) were also prepared. HLF were cultured for 24 h with the three types of conditioned media (IL3IgG, IL5IgG and IL3), a control medium (Ø) and medium including 1 ng/ml of recombinant human IL-3 plus 0.5 μg/ml of HA-IgG (rhIL3IgG). RT-qPCR were performed from total mRNA extracted from two HLF lines (L20 and L21) cultured in the five different conditions. For each HLF lines, controls (Ø) and rhIL3IgG are n = 2. IL3, IL3IgG and IL5IgG conditioned media were prepared from three different eosinophil donors, including two donors previously used for the RNAseq analyses. IL3, IL3IgG and IL5 were compared using One Way Anova (n = 3), and * indicates that IL3IgG is upregulated compared to IL3 and IL5IgG; and # indicates that IL5IgG is upregulated compared to IL3

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Respiratory Research

ISSN: 1465-993X

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