Fig. 4

IL-4 induced caveolin-1-containing lipid rafts aggregation and TRPC1 activation. BECs were treated with 20 ng/ml IL-4 for 24 h, then intracellular co-localization of caveolin-1 and lipid rafts were detected with immunofluorescence (a); the percents of positive lipid rafts aggregation cells and caveolin-1 co-localization cells were calculated (b); caveolin-1 distribution in lipid raft were measured by sucrose gradient centrifugation (c); the ratio of caveolin-1 distribution in lipid raft to non-lipid raft was evaluated (d); intracellular co-localization of caveolin-1 and TRPC1 were detected with immunofluorescence (e); the percents of positive TRPC1 and caveolin-1 co-localization cells were calculated (f); TRPC1 distribution in lipid raft was measured by sucrose gradient centrifugation (g); the ratio of TRPC1 distribution in lipid raft to non-lipid raft was evaluated (h). White arrow (in a and e) indicated lipid rafts aggregation, calveolin-1 aggregation, or TRPC1 aggregation as well as their co-localization respectively. Results are expressed as mean ± SD; n = 4 experiments. *P < 0.05 versus normal control, ^# P < 0.05 versus IL-4 treated group