Fig. 6

Structural and functional impact of rs3746444 variant in MIR-499A gene. a) Genomic location of the SNP on both strands. It represents the nucleotide number 73 (out of 122) of MIR499A on the forward strand (n.73A > G) within the sequence of miR-499a-3p and the nucleotide number 25 (out of 73) of MIR499B on the reverse strand (n.25 T > C) within the sequence of miR-499b-5p. (b) Predicted folding pattern of precursor stem loop structure by RNAfold and KineFold web-servers. No effect of the alleles on the folding pattern was detected. (c) Targets of A allele in miR-499a-3p and miR-499b-5p. (d) Predicted functional effects of G allele on gene targets and gene ontology. Being in the seed region at the 5′ end of miR-499a-3p generates the possibility of creating an altered target gene set for that particular. Using PolymiRTS Database 3.0 platform, identified the disruption of 667 (41.8%) genes of the miR-499a targets and creation of new 763 genes when A allele is substituted by G at the seed region sequence: AAC[A/G]UCACAGCAAGUCUGUGCU