Morphometric Parameters | C3H/HeJ | JF1/MsJ | P value | C3H/HeJ | JF1/MsJ | p value |
---|---|---|---|---|---|---|
Number of mice | MALE: n = 5 | FEMALE: n = 3–5 | ||||
Age (weeks) | 13 | 12–14 | 13 | 12–14 | ||
Fixed lung volume (μl) | 1092 ± 123 | 735 ± 71 | 0.001 | 987 ± 54 | 667 ± 23 | 0.001 |
Mean chord length (Lcm) (μm) | 74.3 ± 9.9 | 48.5 ± 5.5 | 0.002 | 69.2 ± 14.8 | 47.3 ± 2.0 | 0.011 |
Mean alveolar volume (103/μm3) | 61.1 ± 7.9 | 40.8 ± 5.9 | 0.01 | 71.5 ± 11.4 | 42.3 ± 7.0 | 0.007 |
Alveolar number density (106/cm3 parenchyma) | 10.2 ± 1.1 | 14.7 ± 2.0 | 0.005 | 8.8 ± 1.5 | 14.5 ± 2.2 | 0.001 |
Alveolar septum width (μm) | 0.72 ± 0.07 | 0.66 ± 0.08 | ns | 0.82 ± 0.14 | 0.63 ± 0.11 | 0.005 |
Total number of alveoli (106/both lungs) | 9.2 ± 1.1 | 8.9 ± 1.7 | ns | 6.9 ± 1.4 | 8.2 ± 1.6 | ns |
Total alveolar surface area (cm2) | 952 ± 88 | 957 ± 125 | ns | 847 ± 70 | 906 ± 102 | ns |
Elastin - collagen ratio | 1.40 ± 0.3 | 1.95 ± 0.3 | 0.004 | 1.83 ± 0.3 | 2.12 ± 0.2 | 0.035 |
Absolute volumes | ||||||
Parenchymal volume (mm3) | 933 ± 112 | 629 ± 72 | 0.001 | 849 ± 71 | 571 ± 28 | 0.001 |
Non-parenchymal volume (mm3) | 145 ± 33 | 98 ± 23 | 0.002 | 126 ± 22 | 78 ± 11 | 0.003 |
Alveolar airspace volume (mm3) | 574 ± 73 | 377 ± 53 | 0.001 | 514 ± 41 | 340 ± 21 | 0.001 |
Alveolar duct airspace volume (mm3) | 261 ± 32 | 167 ± 26 | 0.041 | 237 ± 28 | 155 ± 15 | ns |
Alveolar septal volume (mm3) | 69 ± 12 | 63 ± 5 | ns | 69 ± 11 | 58 ± 5 | ns |
Total alveolar surface density (μm2/μm3) | 0.10 ± 0.01 | 0.15 ± 0.01 | ns | 0.11 ± 0.01 | 0.16 ± 0.01 | ns |
Alveolar epithelial type I cells (mm3) | 19.5 ± 3.3 | 19.2 ± 3.2 | ns | 20,8 ± 4.02 | 15.5 ± 3.45 | ns |
Alveolar epithelial type II cells (mm3) | 6.0 ± 2.8 | 7.8 ± 4.6 | ns | 4.3 ± 1.5 | 6.5 ± 2.7 | ns |
Capillary endothelial cells (mm3) | 16.3 ± 3.7 | 13.6 ± 1.4 | ns | 15.8 ± 1.9 | 11.8 ± 2.5 | ns |
Interstitial tissue (mm3) | 22.7 ± 5.3 | 17.5 ± 2.1 | ns | 22.5 ± 4.5 | 19.3 ± 6.1 | ns |
Total alveolar surface area (cm2) | 952 ± 88 | 957 ± 125 | ns | 847 ± 70 | 906 ± 102 | ns |
Volume densities | ||||||
Alveolar epithelial type I cells (mm3/mm3) | 28.2 ± 1.5 | 30.5 ± 4.2 | ns | 30.2 ± 2.1 | 27.23 ± 2.4 | ns |
Alveolar epithelial type II cells (mm3/mm3) | 9.0 ± 4.2 | 12.5 ± 6.7 | ns | 6.3 ± 3.4 | 10.9 ± 4.0 | ns |
Capillary endothelial cells (mm3/mm3) | 23.3 ± 2.3 | 21.7 ± 3.1 | ns | 22.8 ± 1.9 | 21.2 ± 2.5 | ns |
Interstitial tissue (mm3/mm3) | 32.9 ± 3.7 | 28.2 ± 2.6 | ns | 32.5 ± 3.0 | 33.2 ± 3.1 | ns |
Collagen (mm3/mm3) | 2.78 ± 0.49 | 2.63 ± 0.51 | ns | 3.02 ± 0.62 | 2.42 ± 0.41 | ns |
Elastin (mm3/mm3) | 3.87 ± 1.23 | 5.08 ± 1.06 | ns | 5.3 ± 1.55 | 5.08 ± 0.72 | ns |
Lung function | n = 4 | n = 3 | n-3 | n-3 | ||
Total Lung capacity (TLC) (μl) | 1425 ± 164 | 750 ± 50 | <0.05 | 1467 ± 125 | 800 ± 82 | <0.05 |
Compliance air (μl/cm H20) | 143 ± 5 | 36 ± 7 | <0.05 | 130 ± 6 | 34 ± 6 | <0.05 |
Compliance saline (μl/cm H20) | 306 ± 46 | 96 ± 13 | <0.05 | 296 ± 37 | 107 ± 20 | <0.05 |
Specific compliance saline (μl/cm H20) | 214 ± 15 | 128 ± 5 | <0.05 | 202 ± 9 | 134 ± 23 | <0.05 |
Compliance saline/Compliance air | 2.2 ± 0.4 | 2.7 ± 0.1 | <0.05 | 2.3 ± 0.2 | 3.2 ± 0.9 | <0.05 |