Fig. 3
a: frequency-response curves (FRC) obtained in 48 bovine trachealis from 6 animal in the absence or presence of different GLY concentrations. Data (means ± SD) are expressed as percent of 64-Hz-induced contraction obtained for each trachealis strip during the first FRC. b: Schild regression to exogenous ACh obtained on 56 bovine trachealis from 7 animals. Regression line was calculated by linear regression analysis of mean values (x) obtained in 7 different experiments. Dashed lines define the 95% confidence limits and arrow is referred to the GLY pA2 value. The dose ratio is the concentration of ACh inducing half-maximal contraction (EC50) in the presence of a given concentration of GLY divided for the EC50 for ACh in the absence of GLY. GLY inhibited both exogenous ACh- and electrically-evoked contractions