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Fig. 1 | Respiratory Research

Fig. 1

From: Fibrocyte measurement in peripheral blood correlates with number of cultured mature fibrocytes in vitro and is a potential biomarker for interstitial lung disease in Rheumatoid Arthritis

Fig. 1

Gating strategy for quantification of fibrocytes using lysed peripheral blood. Representative flow cytometry analysis showing the gating strategy. Population was initially gated on the basis of the forward side scatter characteristics and debris were eliminated, then lymphocyte and monocytes were selected, the CD45+ cells were gated in the plot “CD45-V500” versus forward scatter. Hereafter unwanted cells as B-Cells (CD19+), T-Cells (CD3+), basophils and eosinophils (CD294+) and dead cells (7AAD+) were removed using a PerCP-Cy5-5A dump channel. Finally, the CD45+ CD34+ Cd11b+ fibrocyte population was measured in the “CD11b-Pacific blue versus CD34-PE” plot. Fluoroscence minus one (FMO) controls are shown at the bottom

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Respiratory Research

ISSN: 1465-993X

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