Fig. 2

Detection of IAV in infected lungs. RNA was extracted from infected and uninfected lungs on days 4, 10, and 25, and subjected to RT-PCR using primers specific for the IAV matrix gene. Data represent three separate experiments (2–6 mice per group per day, 26 mice). On day 4 post-infection, the natural log viral gene copies in IAV-infected mice that were administered sirolimus were similar to those in mice received IAV alone. On day 10 post-infection, the viral gene copies were significantly higher in IAV-infected mice that were administered sirolimus than that in mice received IAV alone. Viral gene copies were not detected on day 25 in both groups