Fig. 2
ASCs infusion on engraftment and IDO expression. C57BL/6 mice were transplanted heterotopically with tracheas from C57BL/6 (isograft) or BALB/c (allograft). a 1 × 106 ASCs labeled with Vybrant™ DiD or PBS (0.2 ml) were administered to the recipient mice via tail veins immediately after allograft transplantation. Grafts were harvested and analyzed for the presence of labeled ASCs 24 h later via flow cytometry. b In the allograft group, 1 × 106 human ASCs or PBS were administered to the recipient mice via tail veins on days 0, 1, 3, and 5 post-transplant. Tracheal grafts were harvested on day 7 post-transplant. Expression of IDO protein levels in the grafts were determined via Western blot analysis. The levels of GAPDH expression were assessed as loading controls. All data are expressed as mean ± SEM; n = 5 per group. *, p < 0.05